Yay went right!

Well went back up, tested OD₆₀₀, 0.2 something so waited for anna and had lunch with marv and chin.... i managed to leave my bacteria growing for an hour.... luckily for me when i rushed up the labs to test the OD it was at 0.530 perfect! What great luck! The other was still at 0.3xx so left them to grow some more..... harvested those at 0.479. Put them on ice and followed the procedure, managed to finish at 4:30. Yay! My only concern was that yesterday i used the centrifuge and today i was told i was meant to have used this other one, hope they both end up ok, i dont want to use another day repeating this really boring step!! THe other problem is i didnt put them on ice when i was re-suspening them, whats the problem? The bactiera MIGHT have been growing during the time that they were left out (luckily that was only for a few mins) and then they wont beat the right OD, so maybe left the growth phase.....

Dispite not achieving much today i am still happy for some reason, because it worked (i HOPE!)

Well i will be using them tomorrow, guess i will find out then, no point in worrying!