Sleepy monday

I slept at midnight and so really tired today for some weird reason.... need to sleep earlier (and ocne a upon a time 12 was reasonable) stoopid 9am starts. Practically wasted today (yet again) - i was suppose to do a ligation and then transformation using the EcoR1 digested inserts i prepared on friday..... somehow i threw them away..... i blame that on my purging of non-viable competent cells - my digested insert must have got mixed up with them... and in the bin it went. :'( SO had to re-do that..... ligation takes 4 hours (min.) and didnt manage to start it until 12:30, so didnt have time to do transformation which takes over 1 hour as it needs to be incubated..... yeah little achievements today.

Xi also visited, i am sure one of her blogs will give more details.