Plasmid prep
As each day goes on i seem to be plodding along at a slow and steady pace... it does not seem like i do much, there is a lot of waiting time. I cant seem to work in those short breaks either, by the time i get down to comp lab and start focusing on work, er its time to go back up again!
Summary of todays accomplishments: spin cells down, purified plasmid from cells and run on agrose gel; cut open the vectors with restriction enzymes (vector prep)and run on agrose gel. The rest will be frozen ready for ligating in my GST PCR products tomorrow.
I dont think i will be out of lab until 5:30 still need to wait 10 more mins for vector prep and then run gel (30 mins).
Its now official - the title of my FYP is:Construction of a glutathione-S-transferase expression vector and determination of its effect on biocide degradation
Second marker: Ramesh Wigneshwereraj (Biology). No idea who he is, just hoping he isnt too harsh!
posted by Wink @ 4:23 PM
3 comments
3 Comments:
Jeff said that we're not allowed to work after 5pm. But he did let me stay till 5.30pm to take my last reading of the day.
Well Jeff would be right. I was also told by Steve that we werent suppose to work after 5, although Marv and Xiao seem to be consistently.....
There's probably been an email sent round the supers then. Perhaps their supers weren't super enough and so are not on the super mailing list for super supers.
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