My luck has finally ran out

The day started off badly, i woke up late, so got into uni 20-30 mins late. Boo. And then from there it was downhill. I had to grow up E.coli (cloning and expression cultures) in LB and LB+Tet. It took forever to grow them up. I also had to get a OD600 of 0.5.... thats the difficult part! I managed ti get 0.522 and 0.526 for one of the cultures.... the other culture (expression) i overshot and the other failed to grow! Boo. IF this had worked i would have had a day off to do my literature review!!! Boo boo boo!!!!!!!!!!!! So i have to come in and repeat all this!!

The bacteria just didnt want to grow, i didnt have the right density (0.5 at OD600) until 5pm.... i still had seven other steps to do before i could go home!! I left at 6pm!! Boo!

The incompetenet at lab perevoso boy is finally showing himself.... i wondered how long my luck would last!!